Proteomics can be defined as the large-scale systematic study of proteins expressed in a cell, tissue, organ or organism. The study usually involves separation, quantitation and identification of the proteins. The profile of all proteins present in a system is known as the proteome. Since protein expression levels are varying with time any proteomics study is a snapshot of that specific time and conditions. In higher organisms, studying all the proteins present is highly challenging; therefore targeted proteomics approaches are common. Proteomics research may be targeted towards sub-cellular compartments, organelles, or certain protein types. The proteomics (2D gel electrophoresis) workflow can be divided into ten steps. Each step will be discussed briefly along with our lab facilities. Protein extraction For additional information, contact Victor Asirvatham. Updated October 2006 by Dianne Parkerson | |